abstract：:This report describes a new MHC class II allele, HLA-DR beta 1*0306, discovered in a 31-year-old Norwegian male. The allele typed serologically as DRw52 (DR3) and amplified in PCR using DR52-associated group primers. This product could not be identified using established restriction digests, however. Use of Asp 700, M...

journal_title：Human immunology

authors： Fenske B,Lemieux J,Hoar D,Fitzpatrick J,Keown P

更新日期：1996-03-01 00:00:00

abstract：:A novel cell surface molecule, DN4, defined by an mAb raised against human thymic epithelial cells, showed a specificity for epithelial cells of the thymic cortex. This antigen was not expressed at detectable levels on any other types of tissues in the human body except for the thymus and bone marrow. Immunohistochemi...

journal_title：Human immunology

authors： Chung DH,Choi EY,Lee GK,Bae YM,Hahn JH,Kim SS,Song HG,Park WS,Ahn HS,Park SH

更新日期：1996-02-01 00:00:00

abstract：:HLA analysis of the family of a renal transplant patient revealed an extremely rare condition. On repeated typings the only demonstrable HLA antigens shown in the propositus were from the maternal haplotype, HLA-A11,-B46,-CW1,-DR14,-DQ1. No paternal antigens could be demonstrated either by serologic or by DNA-typing m...

journal_title：Human immunology

authors： van den Berg-Loonen EM,Savelkoul P,van Hooff H,van Eede P,Riesewijk A,Geraedts J

更新日期：1996-01-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) infection has been associated with enhanced expression of HLA antigens on the endothelium and with cellular infiltrates within the graft following human organ transplantation. We investigated the interactions between human cytomegalovirus-infected cultured endothelial cells and cocultured ...

journal_title：Human immunology

authors： Scholz M,Blaheta RA,Cinatl J,Encke A,Doerr HW,Markus BH

更新日期：1995-11-01 00:00:00

abstract：:The purpose of this study was to identify polymorphic residues of DQ2 beta chains which are involved in the epitopes recognized by DQ2-reactive mAbs. Binding studies were performed on a panel of DQ2 mutant cells made by transfection of a DQ (alpha 1*0501, beta 1*0301)-positive B-LCL with DQB1 genes encoding either wil...

journal_title：Human immunology

authors： Viken HD,Paulsen G,Drover S,Marshall WH,Sollid LM,Gaudernack G,Thorsby E

更新日期：1995-10-01 00:00:00

abstract：:The human TCRBV gene complex contains three BV12 genes and all are polymorphic. There are two alleles of BV12S4; one allele contains a stop codon in the coding region which precludes its expression. There are four alleles of BV12S2 and two alleles of BV12S3 genes; however, the substitutions present in both genes are c...

journal_title：Human immunology

authors： Deulofeut H,Wilderson JL,Barron KS,Robinson MA

更新日期：1995-07-01 00:00:00

abstract：:The influence of subtle HLA diversification on antigen binding was explored using murine L-cell transfectants expressing alleles in the DR11/DR13 family and a panel of peptides. The levels of binding among this family of DR microvariants were as diverse as the levels of binding among distantly related DR molecules. Ev...

journal_title：Human immunology

authors： Hurley CK,Steiner N

更新日期：1995-06-01 00:00:00

abstract：:IDDM is known to be associated with genes of HLA complex, particularly alleles of HLA-DQ. The 40-kb TAP gene complex is located approximately 150 kb centrometric to the DQB1 locus. The TAP1-TAP2 protein heterodimer is required for normal expression levels of class I, molecules on the surface of cells. While present ev...

journal_title：Human immunology

authors： Jackson DG,Capra JD

更新日期：1995-05-01 00:00:00

abstract：:To investigate lymphocyte subpopulations and the status of T-cell activation at different phases of the menstrual cycle, lymphocytes in endometrial tissue were analyzed by dual-color flow cytometry in 39 patients. Compared with peripheral blood, the lymphocytes in the endometrium had a higher CD3-/(CD56+ or CD16+) rat...

journal_title：Human immunology

authors： Chen CK,Huang SC,Chen CL,Yen MR,Hsu HC,Ho HN

更新日期：1995-03-01 00:00:00

abstract：:We previously investigated the characteristics of renal allograft infiltrating T-cell lines that were propagated from biopsy and nephrectomy specimens designated as IG-Bip and IG-Neph, respectively, or analogous line designated IG-T-eff, which was generated by co-culturing pretransplant recipient blood with irradiated...

journal_title：Human immunology

authors： Emara MA,Mozes MF

更新日期：1995-02-01 00:00:00

abstract：:PCR-based analyses were performed for the identification of HLA-B44 subgroups. Genomic DNA from six homozygous cell lines and 44 healthy individuals who had serologically tested positive for HLA-B44 was investigated for polymorphism in exons 2 and 3 of the HLA-B44 genes. Two primers were designed for specific amplific...

journal_title：Human immunology

authors： Yao Z,Keller E,Scholz S,McNicholas A,Volgger A,Albert ED

更新日期：1995-01-01 00:00:00

abstract：:The association of HLA antigens and type I or "lupoid" CAH-C was investigated in a population of 52 Argentinian Caucasoid patients. When compared with a population of normal individuals of the same ethnic group (n = 197), a significant increase of HLA-DR6 was observed (68.6% in patients vs 17.3% in controls; RR = 12.3...

journal_title：Human immunology

authors： Fainboim L,Marcos Y,Pando M,Capucchio M,Reyes GB,Galoppo C,Badía I,Remondino G,Ciocca M,Ramonet M

更新日期：1994-10-01 00:00:00

abstract：:The proteins synthesized in the cytosol are several thousand, and the number of peptides potentially able to be bound by class I molecules they can generate is therefore huge. On the other hand, the actual number of peptide-class I complexes required for CTL activation is around 200. We focused on the peptides bound b...

journal_title：Human immunology

authors： Frumento G,Molina F,Pernis B,Ferrara GB

更新日期：1994-09-01 00:00:00

abstract：:The contributions of individual amino acids in the polymorphic beta chain and the conserved alpha chain of HLA-DR1 to influenza HA-specific DR1-restricted and anti-DR1 allospecific T-cell recognition were analyzed. The genes encoding HLA-DR1 were subjected to site-directed mutagenesis in order to introduce single amin...

journal_title：Human immunology

authors： Tuosto L,Karr RW,Fu XT,Olson RR,Cundari E,Piccolella E,Lechler R,Lombardi G

更新日期：1994-08-01 00:00:00

abstract：:Beta 2m serum levels have been shown to be increased in patients with tuberculosis and HIV infection. We determined the stability of beta 2m and of sHLA-I dimers in serum, and then determined the levels of both molecules in 60 non-HIV-infected patients with active pulmonary tuberculosis and in 55 adult controls. The l...

journal_title：Human immunology

authors： Inostroza J,Muñoz P,Espinoza R,Millaqueo L,Diaz P,Leiva L,Sorensen R

更新日期：1994-07-01 00:00:00

abstract：:Using TCR-specific mAbs, we analyzed the TCR distribution in the CD4 and CD8 compartments of peripheral blood T cells from members of six families, including two which were informative for an HLA-B/DR crossover. TCR distribution in the CD4 compartment is influenced by MHC class II antigens as it was similar in the chi...

journal_title：Human immunology

authors： Reed EF,Tugulea SL,Suciu-Foca N

更新日期：1994-06-01 00:00:00

abstract：:A comprehensive system for genetic typing of the HLA class I A locus is described, based on PCR amplification and typing with nonradioactively labeled SSO probes. Exons 1-3 of the A locus are amplified and typing is performed with a set of 30 nonradioactively labeled oligonucleotide probes. This system resolves 34 of ...

journal_title：Human immunology

authors： Allen M,Liu L,Gyllensten U

更新日期：1994-05-01 00:00:00

abstract：:Nucleotide sequences of alleles encoding four serologically defined splits of the HLA-A10 group, A26.1, A26.3, A26.4, and A10SA, were determined. It was confirmed that the alleles coding for A26.1 and A26.3 are identical with A*2602 and A*2601, respectively. On the other hand, alleles for A26.4 and A10SA are thus far ...

journal_title：Human immunology

authors： Ishikawa Y,Tokunaga K,Lin L,Imanishi T,Saitou S,Kimura A,Kashiwase K,Akaza T,Tadokoro K,Juji T

更新日期：1994-03-01 00:00:00

abstract：:Polymorphism in the URR of the MHC class II DQA1 gene defines ten different alleles named QAP. Oligotyping for the alleles of DRB1, QAP, DQA1, and DQB1 have been performed in 210 unrelated healthy controls from Germany. Moreover, 83 HTCs from the Tenth IHWS have been tested. Four point loci haplotypes (DRB1, QAP, DQA1...

journal_title：Human immunology

authors： Haas JP,Kimura A,Andreas A,Hochberger M,Keller E,Brünnler G,Bettinotti MP,Nevinny-Stickel C,Hildebrandt B,Sierp G

更新日期：1994-01-01 00:00:00

abstract：:To simplify PCR-SSO HLA-DRB generic typing, we labeled eight of 15 oligonucleotide probes with DIG and the others with biotin, and hybridized each dot blot with both a biotin-labeled probe and a DIG-labeled probe simultaneously. We chose oligonucleotide pairs which require the same hybridization and stringent washing ...

journal_title：Human immunology

authors： Chen DF,Endres W,Meyer SA,Stangel W

更新日期：1994-01-01 00:00:00

abstract：:MHC class II molecules play a central role in the control of the immune response, but their biologic function and mechanism of action on the surface of activated human T lymphocytes are not entirely understood. In our study, the functional role of HLA class II molecules in T-blast proliferation was investigated by ana...

journal_title：Human immunology

authors： Di Rosa F,D'Oro U,Ruggiero G,Racioppi L,Acquaviva A,Ferrone S,Fontana S,Zappacosta S

更新日期：1993-12-01 00:00:00

abstract：:RING 11, a second transport-associated gene (TAP2), has been recently identified in the DR-DP interval of the human class II region. Two predominant alleles, TAP2A and TAP2B, differing by 17 amino acids at the C-terminus of the ATP-binding domain are present in the Caucasoid population at frequencies of 79% and 21%, r...

journal_title：Human immunology

authors： Eiermann TH,Fakler J,Goldmann SF,Böhm BO

更新日期：1993-11-01 00:00:00

abstract：:HLA-B703 (BPOT) is a B7-like antigen segregating within families. To determine the complete B*0703 nucleotide sequence, HLA-ABC-cDNAs derived from the EBV cell line POT71 have been cloned. Four B*0703-positive clones were sequenced to obtain an accurate consensus sequence. With respect to the isoelectric focusing-iden...

journal_title：Human immunology

authors： Bergmans AM,Tijssen H,Lardy N,Reekers P

更新日期：1993-10-01 00:00:00

abstract：:The MHC in humans is a much studied region of the genome, the genes of which display a high rate of polymorphism and a high rate of linkage disequilibrium. Four families in which intra-class-I recombination has occurred have been analyzed with six polymorphic markers between HLA-A and -B in order to determine the full...

journal_title：Human immunology

authors： Crouau-Roy B,Bouissou C,Sommer E,Pontarotti P,Thomsen M

更新日期：1993-10-01 00:00:00

abstract：:HLA-DQA1 typing of the 4AOHW cell panel is presented using a novel strategy that exploits both intron and exon polymorphisms. Intron sequences adjacent to the variable HLA-DQA1 second exon exhibit stable polymorphisms that are specific for locus alleles and certain suballelic DR/DQ haplotypes. A PCR-RFLP method has be...

journal_title：Human immunology

authors： Limm TM,Ashdown ML,Naughton MJ,McGinnis MD,Simons MJ

更新日期：1993-09-01 00:00:00

abstract：:Experiments were carried out to determine whether complexes between MHC class I molecules and synthetic peptides are representative of those formed under more physiologically relevant conditions, with peptides derived intracellularly from processed antigens. Lysis of cells sensitized with exogenously provided and endo...

journal_title：Human immunology

authors： Sauma SY,Gammon MC,Bednarek MA,Cunningham B,Biddison WE,Hermes JD,Porter G,Tamhankar S,Hawkins JC,Bush BL

更新日期：1993-08-01 00:00:00

abstract：:Routine HLA typing by serology has been supported by DNA or protein analysis of the respective molecules in cases when serologic typing was inconclusive or difficult to perform. DNA analysis by RFLP, SSO, or PCR amplification with SSP is a reliable tool for the identification of alleles. Because DNA sequences may now ...

journal_title：Human immunology

authors： Rozemuller EH,Bouwens AG,Bast BE,Tilanus MG

更新日期：1993-08-01 00:00:00

abstract：:TCR V alpha 3 and V alpha 5 transcripts in PBLs from healthy individuals of multiple age groups and from BMT recipients were analyzed. PCR, cloning, and sequencing studies revealed significant V-J junctional diversity among TCR transcripts from all tested blood samples, as provided both by N/P-region addition and exon...

journal_title：Human immunology

authors： Dave VP,Larché M,Rencher SD,Koop BF,Hurwitz JL

更新日期：1993-07-01 00:00:00

abstract：:Mononuclear cells obtained from a child at the acute presentation of type I diabetes were stimulated in vitro with human insulin followed by IL-2 and IL-4. All of the T-cell clones isolated from this stimulation were autoreactive, recognizing autologous B cells in the absence of insulin or other exogenous antigens. El...

journal_title：Human immunology

authors： Miller G,Nepom GT,Reich MB,Thomas JW

更新日期：1993-04-01 00:00:00

abstract：:To clarify the molecular relationship between HLA loci and ulcerative colitis (UC) in Japanese patients, we performed HLA-DP genotyping by the PCR-RFLP method and studied tumor necrosis factor beta-chain genetic polymorphism by Southern hybridization, in addition to conventional serologic typing. Significant increase ...

journal_title：Human immunology

authors： Sugimura K,Asakura H,Mizuki N,Inoue M,Hibi T,Yagita A,Tsuji K,Inoko H

更新日期：1993-02-01 00:00:00

abstract：:In an attempt to develop a suitable model for increasing the yield of human anti-HLA mAbs, we have used mice with SCID for i.p. injection of two human-mouse heterohybridomas. HMP1 hybridoma secretes a DQB1*0201 allele-specific human mAb whereas HMP12 secretes a human mAb recognizing the DRB1*1101, 1102, 1103, and 1104...

journal_title：Human immunology

authors： Pistillo MP,Sguerso V,Ferrara GB

更新日期：1992-12-01 00:00:00

abstract：:We describe the production of mouse monoclonal antibodies specific for the human TcR using as the immunogen transfected murine T-cell hybridoma cells coexpressing mouse CD3 with human Jurkat TcR alpha and beta chains. The shortage of monoclonal antibodies (mAbs) specific for the human TcR-V alpha and V beta families r...

journal_title：Human immunology

authors： Zumla A,McCormack A,George A,Batchelor R,Lechler R

更新日期：1992-11-01 00:00:00

abstract：:The class II MHC gene DPA is inducible by interferon-gamma (IFN-gamma), whereas the DQB gene is not inducible in most cell types. To investigate the DNA region specifically responsible for inducibility or its lack that may be required (in addition to the elements required for constitutive expression of class II genes)...

journal_title：Human immunology

authors： Sugawara M,Ponath PD,Yang Z,Strominger JL

更新日期：1992-11-01 00:00:00

abstract：:Detection and avoidance of donor-reactive antibodies in the sera of potential organ transplant recipients is key to a successful transplant outcome. Techniques of antibody detection that use flow cytometry are more sensitive than those that rely upon a visual determination of cytotoxicity. However, as conventionally p...

journal_title：Human immunology

authors： Wetzsteon PJ,Head MA,Fletcher LM,Lye WC,Norman DJ

更新日期：1992-10-01 00:00:00

abstract：:In the present study, we explored the specific requirements for lysis of human activated T cells by CD4+ CTLs. This was achieved by using human CD4+ T cell lines or clones specific for a peptidic fragment of influenza virus as both CTL effectors and target T cells (TTCs). Our results further establish that human activ...

journal_title：Human immunology

authors： Eljaafari A,Dorval I,Zeliszewski D,Le Gac S,Sterkers G

更新日期：1992-09-01 00:00:00

abstract：:There are four DRw8 haplotypes with different DQ alleles in Japanese: DRw8-DQw6 (w1), DRw8-DQw4, DRw8-DQw8(w3), and DRw8-DQw7 (w3). We previously reported the nucleotide sequence of DRB1 gene of DRw8-DQw6(w1) and it was named DRB1*08032. The nucleotide sequences of the other DRw8 DRB1 alleles and their correspondence ...

journal_title：Human immunology

authors： Watanabe Y,Matsushita S,Kohsaka T,Kobayashi N,Nose Y,Tokunaga K,Juji T

更新日期：1992-07-01 00:00:00

abstract：:Recent studies from this laboratory reported the mapping of the full profile of T-cell allorecognition regions of HLA-DR2 beta subunit. The results indicated the presence of an allodeterminant within DR2 beta regions 141-156. In another study, we have shown that this allodeterminant is one of five regions of structura...

journal_title：Human immunology

authors： Atassi H,Atassi MZ

更新日期：1992-05-01 00:00:00

abstract：:Peptides derived from mutated human proto-oncogenes bound to HLA may represent a novel type of tumor-specific antigen. Mutated ras genes are the oncogenes most frequently identified in human cancer. The transforming genes carry a mutation in codons 12, 13, or 61. We have investigated whether the T-cell repertoire of h...

journal_title：Human immunology

authors： Gedde-Dahl T 3rd,Eriksen JA,Thorsby E,Gaudernack G

更新日期：1992-04-01 00:00:00

abstract：:During HLA-DQ oligotyping, the presence of a novel HLA-DQB1 allele was suggested by a pattern of probe hybridization that was inconsistent with the presence of any possible combination of two known alleles. The nucleotide sequence of the second exon of the HLA-DQB1 gene was determined, revealing a novel HLA-DQB1 seque...

journal_title：Human immunology

authors： Fenske TS,Baxter-Lowe LA

更新日期：1992-03-01 00:00:00

abstract：:A simple method for obtaining DNA from serum and plasma is described. Using appropriate primer pairs the polymorphic segments of HLA class II genes were amplified by the polymerase chain reaction (PCR) from this DNA, and typed using allele-specific oligonucleotide hybridization. When compared with DNA obtained from pe...

journal_title：Human immunology

authors： Martin M,Carrington M,Mann D

更新日期：1992-02-01 00:00:00